《微生物学实验》是一本选材于实际并具有一定特色的微生物学实验指导教材。它总括了参与昆明学院微生物学实验及理论教学的几位老师在多年的微生物学教学、科研及生产应用方面的宝贵经验，吸收了同类教材及研究手册的优点，为适应当今生物科技的发展、贴合微生物学理论教学编写而成。
全书共包括15个经典的微生物学实验，图文并茂地介绍了培养基的配制与灭菌消毒技术，环境微生物检测，显微镜使用技术，各类微生物的制片及形态结构观察，各类微生物的分离、纯化和培养，微生物生理生化反应，微生物生长等内容。着重训练学生微生物学实验的基本操作和技能，同时适当增加了一些探究性问题，引导学生在掌握基础实验内容后，去思考如何将实验与理论知识及具体实践应用相结合的问题。全书每个实验中都附有相关染色液及试剂等的配制方法，取材尽量贴合实际环境及条件，方便学生实验操作。在每个实验内容后还引入了延伸阅读部分，主要介绍一些和实验相关的趣闻、新的研究进展及相应实验在实际生活中的应用，这加深了相关实验内容的广度和深度，体现出一定的实用性和灵活性，也便于提起学生学习兴趣，引导学生课后主动查阅更多的与实验相关的知识。
本书可供高等院校生物科学专业、生物技术专业、发酵工程、农学等专业的大学本、专科学生使用，也可供从事微生物学及相关学科教学及生产的科技工作者参考使用。（It is an experimental textbook of microbiology experiment based on reality with certain characteristics.It sums up the years of experience of microbiology teaching, scientific research and production application of teachers involved in instructing microbiology experiment and theory at Kunming University, absorbs the advantages of similar materials and research manuals in order to adapt to the development of modern biotechnology and conform to the teaching of microbiology theories.
The book includes 15 classical microbiology experiments with rich illustrations to introduce the preparation and sterilization disinfection technology of culture medium, environmental microbial detection, microscopy usages, all kinds of microbe production and morphology observation, isolation, purification and culture of all kinds of microorganisms, microbial physiological and biochemical reaction and microbial growth, etc.The book focuses on training students the basic operation and skills of microbiology experiment, and at the same time adds some exploratory problems to guide the student who have mastered basic experiment content to think about how to combine experiment and theoretical knowledge with practice and application.Each experiment is equipped with the preparation method of dyeing liquid and reagent, which can be used to fit the actual environment and conditions to facilitate students' experimental operation.After each experiment there is extended reading which mainly introduces some related to the experiment, the latest research progress and their corresponding application in the real life, which can enhance the scope and depth of related experiments and reflect certain practicability and flexibility, and also to stimulate interest, and guide students to take the initiative to consult more related experiments after class.
The book can be used by college and junior college students of biological science, biotechnology, fermentation engineering and agronomy and other programs, or used by science and technology professionals engaged in teaching and production of microbiology and related disciplines.）